Human Gc(glucagon) Elisa Kit

Synonyms: Carbohydrate-Rich Portion of Platelet Membrane Glycoprotein Ib Alpha Polypeptide

Product name:Human GC(Glucagon) ELISA Kit

Catalog NO.:FY-EH4508

Reactivity:Human

Assay range:15.6–1,000pg/mL

Sensitivity:<7.5pg/mL

Detection Method:Double-antibody Sandwich

Size:48T, 96T

Storage:2–8 ℃ for 6 months

CV (%):Intra-Assay: CV<8%; Inter-Assay: CV<10%

Note:For Research Use Only

GC(Glucagon) Basic information

Glucagon is a peptide hormone, produced by alpha cells of the pancreas. It raises concentration of glucose and fatty acids in the bloodstream, and is considered to be the main catabolic hormone of the body. It is also used as a medication to treat a number of health conditions. Its effect is opposite to that of insulin, which lowers extracellular glucose. It is produced from proglucagon, encoded by the GCG gene. The pancreas releases glucagon when the amount of glucose in the bloodstream is too low. Glucagon causes the liver to engage in glycogenolysis: converting stored glycogen into glucose, which is released into the bloodstream. High blood-glucose levels, on the other hand, stimulate the release of insulin. Insulin allows glucose to be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels stable. Glucagon increases energy expenditure and is elevated under conditions of stress. Glucagon belongs to the secretin family of hormones.

Human GC(Glucagon) ELISA Kit test method

Feiyue’s Human GC(Glucagon) is an ELISA reagent for detection of GC(Glucagon) in Plasma.

This kit uses sandwich ELISA to detect the concentration of Human GC(Glucagon). Human GC(Glucagon)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human GC(Glucagon) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human GC(Glucagon) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000115263 — Ensembl, May 2017

2. “Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.

3. Voet D, Voet JG (2011). Biochemistry (4th ed.). New York: Wiley.

4. Reece J, Campbell N (2002). Biology. San Francisco: Benjamin Cummings. ISBN 978–0–8053–6624–2.