Precision Separation: HPLC Column

Explore the impact of particle size changes in HPLC columns, instructions for using a new HPLC column, packing procedures, and the method for calculating column volume in High-Performance Liquid Chromatography.

Optimizing HPLC Column Performance

Understand the effects of particle size adjustments, step-by-step guidance for utilizing a new HPLC column, packing procedures, and the formula for calculating column volume in High-Performance Liquid Chromatography.

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Best HPLC Column Comparison Table

Place of Origin

Type
Length
Particle Size
Inner Diameter
Pore Size
Chemistry
Pressure Limit
Temperature Limit
Compatibility
Warranty

United States
- Reversed phase
- 150 - 250 mm
- 3 - 5 µm
  Top 3 - 5 µm Vendors Globally
- 2.1 - 4.6 Mm
  Top 2.1 - 4.6 Mm Vendors Globally
- 100 - 300 Å
  Top 100 - 300 Å Vendors Globally
- C18, C8, C4, Phenyl
  Top C18, C8, C4, Phenyl Vendors Globally
- 400 - 600 Bar
  Top 400 - 600 Bar Vendors Globally
- 40 - 80°C
  Top 40 - 80°C Vendors Globally
- LC/MS, LC/UV
  Top LC/MS, LC/UV Vendors Globally
- 2 years
United Kingdom
- Ion exchange
- 100 - 300 mm
- 2 - 6 µm
  Top 2 - 6 µm Vendors Globally
- 1.9 - 4.5 Mm
  Top 1.9 - 4.5 Mm Vendors Globally
- 80 - 250 Å
  Top 80 - 250 Å Vendors Globally
- Anion, Cation
  Top Anion, Cation Vendors Globally
- 350 - 550 Bar
  Top 350 - 550 Bar Vendors Globally
- 30 - 70°C
  Top 30 - 70°C Vendors Globally
- LC/MS, LC/UV
  Top LC/MS, LC/UV Vendors Globally
- 1 year
Australia
- Size exclusion
- 200 - 350 mm
- 3 - 4 µm
  Top 3 - 4 µm Vendors Globally
- 2.0 - 4.0 Mm
  Top 2.0 - 4.0 Mm Vendors Globally
- 120 - 320 Å
  Top 120 - 320 Å Vendors Globally
- SEC, HIC, GPC
  Top SEC, HIC, GPC Vendors Globally
- 450 - 650 Bar
  Top 450 - 650 Bar Vendors Globally
- 45 - 85°C
  Top 45 - 85°C Vendors Globally
- LC/MS, LC/UV
  Top LC/MS, LC/UV Vendors Globally
- 2 years
Japan
- Affinity
- 120 - 280 mm
- 2 - 5 µm
  Top 2 - 5 µm Vendors Globally
- 1.8 - 4.2 Mm
  Top 1.8 - 4.2 Mm Vendors Globally
- 90 - 280 Å
  Top 90 - 280 Å Vendors Globally
- Protein A, Protein G
  Top Protein A, Protein G Vendors Globally
- 380 - 580 Bar
  Top 380 - 580 Bar Vendors Globally
- 35 - 75°C
  Top 35 - 75°C Vendors Globally
- LC/MS, LC/UV
  Top LC/MS, LC/UV Vendors Globally
- 1 year

Common Questions About HPLC Column

Q: Which statements are true if the particle size in an HPLC column is decreased?

A: Smaller particle sizes enhance resolution and efficiency but may increase backpressure, impacting flow rates and system compatibility.

Q: How to use a new HPLC column?

A: Flush the column with solvent, equilibrate it with the mobile phase, and run a few initial test injections to stabilize performance before analytical use.

Q: How to pack an HPLC column?

A: Follow manufacturer guidelines, ensuring proper packing density and avoiding air bubbles, to achieve uniform and efficient column packing for optimal separation.